anti notch4  (Cell Signaling Technology Inc)

Journal: PLOS ONE

Article Title: Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma

doi: 10.1371/journal.pone.0317709

Figure Lengend Snippet: List of primers for detection of Notch pathway components by qRT-PCR.

Article Snippet: Notch4 , Notch4 transmembrane receptor , Rb , Thermo Scientific, Dreieich, Germany , PA5-40587 , 1:100.

Techniques: Marker, Sequencing

Journal: PLOS ONE

Article Title: Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma

doi: 10.1371/journal.pone.0317709

Figure Lengend Snippet: Overview on antibodies used for detection of Notch pathway components by immunofluorescence and PLA.

Article Snippet: Notch4 , Notch4 transmembrane receptor , Rb , Thermo Scientific, Dreieich, Germany , PA5-40587 , 1:100.

Techniques: Immunofluorescence

Journal: PLOS ONE

Article Title: Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma

doi: 10.1371/journal.pone.0317709

Figure Lengend Snippet: (A) Relative mRNA expression (normalized to housekeeping gene β-actin) of target genes by qRT-PCR in different BCa cell lines; # significance versus non-invasive, papillary RT-4 cells (G1); * significance between G2-G4 cell lines; p ≤ 0.05, One-way ANOVA, Tukey’s multiple comparisons test, mean + SD, n = 3. (B,C) Protein expression of targets by immunofluorescence in different cell lines. (B) Representative immunofluorescence images of Notch1, Notch2, Notch3, Notch4 and DLL4 staining, exemplarily shown for RT-4 cells; scale bar: 20 µm. (C) Quantification of mean fluorescence intensity in CK AE-positive ROIs; # significance versus non-invasive, papillary RT-4 cells (G1); * significance between G2-G4 cell lines; p ≤ 0.05, One-way ANOVA, Tukey’s multiple comparisons test, mean + SD, n = 3.

Article Snippet: Notch4 , Notch4 transmembrane receptor , Rb , Thermo Scientific, Dreieich, Germany , PA5-40587 , 1:100.

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, Fluorescence

Journal: PLOS ONE

Article Title: Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma

doi: 10.1371/journal.pone.0317709

Figure Lengend Snippet: (A,B) Protein expression of canonical downstream molecules by immunofluorescence in different cell lines. (A) Representative immunofluorescence images of HES1 and HEY1 staining, exemplarily shown for RT-4 and KU-19-19 cells; scale bar: 20 µm. (B) Quantification of mean fluorescence intensity in CK AE-positive ROIs; # significance versus non-invasive, papillary RT-4 cells (G1); * significance between G2-G4 cell lines; p ≤ 0.05, One-way ANOVA, Tukey’s multiple comparisons test, mean + SD, n = 3. (C,D) Correlation of HES1 and HEY1 with Notch receptors. (C) Fluorescence intensity (FI) of HES1 show significant correlation to FI of Notch 2, Notch3 and Notch4; (D) HEY1 is significantly related to Notch2 and Notch3; * p ≤ 0.05; Pearson correlation analysis; n = 3.

Article Snippet: Notch4 , Notch4 transmembrane receptor , Rb , Thermo Scientific, Dreieich, Germany , PA5-40587 , 1:100.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence